3G85
Crystal structure of LacI family transcription regulator from Clostridium acetobutylicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.024, 96.058, 99.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.300 - 1.840 |
| R-factor | 0.18623 |
| Rwork | 0.184 |
| R-free | 0.23035 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.081 | 0.403 |
| Number of reflections | 26906 | |
| <I/σ(I)> | 16.3 | 2.89 |
| Completeness [%] | 72.3 | |
| Redundancy | 11.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris pH 6.5, 28% w/v PEG MME 2000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
