3G83
Crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with alpha 1,2 dimannose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.754, 108.774, 56.011 |
Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
Resolution | 25.226 - 1.900 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21500 |
Starting model (for MR) | 1ork |
RMSD bond length | 0.006 |
RMSD bond angle | 0.932 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.500 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.049 | 0.249 |
Number of reflections | 50168 | |
<I/σ(I)> | 15.9 | 6.7 |
Completeness [%] | 94.9 | 90.9 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 150MM NACL, 10MM CACL2, 12% PEG 8000, 100MM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |