3G7Y
Crystal structure of oxidized Ost6L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.031, 59.391, 105.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.310 - 2.215 |
R-factor | 0.2258 |
Rwork | 0.219 |
R-free | 0.27960 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Ost6L structure solved by SAD (bromide signal) |
RMSD bond length | 0.005 |
RMSD bond angle | 0.908 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.310 | 2.300 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 9389 | |
<I/σ(I)> | 18 | 5.9 |
Completeness [%] | 98.9 | 92 |
Redundancy | 7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% PEG 3350, 50 mM Na-citrate, pH 3.0, protein solution buffer: 50 mM Tris-HCl, pH 8.0 mixing ratio 1.5(protein):1(precipitant), VAPOR DIFFUSION, SITTING DROP, temperature 277K |