3G7G
Crystal structure of the protein with unknown function from Clostridium acetobutylicum ATCC 824
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.048, 166.841, 66.974 |
Unit cell angles | 90.00, 119.06, 90.00 |
Refinement procedure
Resolution | 58.620 - 1.990 |
R-factor | 0.19649 |
Rwork | 0.193 |
R-free | 0.25370 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.648 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.620 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.103 | 0.439 |
Number of reflections | 81173 | |
<I/σ(I)> | 12.63 | 1.47 |
Completeness [%] | 97.9 | 84.82 |
Redundancy | 4.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% PEG 3350, 0.1M Bis-tris, 0.2M NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |