3G6R
GR DNA binding domain:FKBP5 complex-52, 18bp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-26 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 1.282963 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.688, 101.310, 112.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.195 - 2.300 |
R-factor | 0.1923 |
Rwork | 0.187 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1glu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.301 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.508 |
Number of reflections | 19913 | |
<I/σ(I)> | 19.3 | 1.88 |
Completeness [%] | 95.1 | 65 |
Redundancy | 11.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 50mM Na Cacodylate, pH 6.5, 2.25mM Spermine, 18mM MgCl2, 9% Isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Cacodylate | ||
2 | 1 | 1 | Spermine | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 2 | Cacodylate | ||
5 | 1 | 2 | Spermine | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | Isopropanol |