3G6P
GR DNA binding domain:FKBP5 complex, 18bp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-10 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 1.115894 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.060, 100.110, 112.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.694 - 1.985 |
R-factor | 0.2155 |
Rwork | 0.214 |
R-free | 0.23890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1glu |
RMSD bond length | 0.008 |
RMSD bond angle | 1.233 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.079 | 0.031 |
Number of reflections | 30730 | |
<I/σ(I)> | 14.03 | 0.38 |
Completeness [%] | 98.0 | 83.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM Na cacodylate pH 6.0, 1mM Spermidine, 20mM MgCL2, 2mM CaCl2, 10% 1,5-hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Cacodylate | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | Spermidine | ||
5 | 1 | 2 | Cacodylate | ||
6 | 1 | 2 | CaCl2 | ||
7 | 1 | 2 | MgCl2 | ||
8 | 1 | 2 | 1,5-hexanediol | ||
9 | 1 | 2 | Spermidine |