3G6N

Crystal structure of an EfPDF complex with Met-Ala-Ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 64 2 2
Unit cell lengths	148.688, 148.688, 141.902
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.225
Rwork	0.225
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmd
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	30468
Completeness [%]	93.6	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	Na-malonate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K