3G6L
The crystal structure of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.088, 71.715, 59.075 |
Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
Resolution | 45.590 - 1.800 |
R-factor | 0.21237 |
Rwork | 0.210 |
R-free | 0.25739 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w9p |
RMSD bond length | 0.015 |
RMSD bond angle | 1.556 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.480 |
Number of reflections | 30889 | |
Completeness [%] | 95.0 | 92.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2M ammonium dihydrogen phosphate, 15%(w/v) PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |