3G5Y
Antibodies Specifically Targeting a Locally Misfolded Region of Tumor Associated EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.176, 69.267, 71.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.180 - 1.590 |
| R-factor | 0.19851 |
| Rwork | 0.196 |
| R-free | 0.25049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.180 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.086 | 0.300 |
| Number of reflections | 43879 | |
| Completeness [%] | 78.8 | 11.8 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.15M Na formate, 15% PEG1500, EVAPORATION, temperature 293K |
