3G5S
Crystal structure of Thermus thermophilus TrmFO in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.90000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.257, 92.226, 104.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.811 - 1.050 |
R-factor | 0.163 |
Rwork | 0.162 |
R-free | 0.17840 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.179 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.850 |
High resolution limit [Å] | 1.050 |
Number of reflections | 209948 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 13% PEG 3350, 0.1M magnesium chloride, 0.05M Tris-HCl (pH 8.5), 10mM reduced-glutathione, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 8.5 | 298 | 13% PEG 3350, 0.1M magnesium chloride, 0.05M Tris-HCl (pH 8.5), 10mM reduced-glutathione, VAPOR DIFFUSION, temperature 298K |