3G5L
Crystal structure of putative S-adenosylmethionine dependent methyltransferase from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-09-30 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.812, 92.532, 99.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.350 |
R-factor | 0.19235 |
Rwork | 0.190 |
R-free | 0.27618 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.199 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.121 | 0.430 |
Number of reflections | 22700 | |
<I/σ(I)> | 5.6 | 2.7 |
Completeness [%] | 90.7 | 99.8 |
Redundancy | 6.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1M Lithium chloride pH 7.5, 100mM Sodium acetate, 30% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |