3G59
Crystal Structure of Candida glabrata FMN Adenylyltransferase in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.793, 79.793, 77.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.870 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fwk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.536 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 24161 | |
| <I/σ(I)> | 21.984 | 2.456 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 289 | 0.1 M Sodium acetate, pH 4.4, 6% (w/v) PEG 4000, Temperature 289K, vapor diffusion, hanging drop |
