3G4H
Crystal structure of Human Senescence Marker Protein-30 (Zinc Bound)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-16 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.365, 52.019, 85.832 |
Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
Resolution | 84.520 - 1.920 |
R-factor | 0.22519 |
Rwork | 0.224 |
R-free | 0.25336 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g4e |
RMSD bond length | 0.035 |
RMSD bond angle | 2.582 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.19) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.520 | 2.020 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.086 | 0.013 |
Number of reflections | 42805 | |
<I/σ(I)> | 5.251 | 0.5 |
Completeness [%] | 99.0 | 94 |
Redundancy | 7 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.8 | 298 | 38% PEG 6000, 0.1M Tris-Hcl, 5mM DTT, 5mM CaCl2, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |