3G4E
Crystal structure of human senescence marker protein-30(SMP30)(Calcium bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.474, 51.001, 86.124 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.420 |
| R-factor | 0.136 |
| Rwork | 0.129 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ghs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | PHASER |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.819 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.063 | 0.808 |
| Total number of observations | 89400 | |
| Number of reflections | 14159 | |
| <I/σ(I)> | 7.056 | 0.8 |
| Completeness [%] | 98.7 | 91.9 |
| Redundancy | 7.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 38% PEG 6000, 0.1M Tris-Hcl, 5mM calcium chloride, 5mM DTT, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
