3G48
Crystal structure of chaperone CsaA form Bacillus anthracis str. Ames
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2008-12-17 |
Detector | ADSC QUANTUM Q315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.359, 67.564, 74.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.820 - 1.500 |
R-factor | 0.16744 |
Rwork | 0.166 |
R-free | 0.20057 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nzh |
RMSD bond length | 0.020 |
RMSD bond angle | 1.745 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.077 | 0.560 |
Number of reflections | 32247 | |
<I/σ(I)> | 20 | 2.1 |
Completeness [%] | 98.8 | 86.7 |
Redundancy | 4.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 12% Peg 20000, 10 % glycerol, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP |