3G3T
Crystal structure of a eukaryotic polyphosphate polymerase in complex with orthophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.918 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 136.110, 136.110, 84.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.647 - 1.850 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g3q |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.300 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 39665 | |
<I/σ(I)> | 28.1 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20.9 | 20.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 1 M Na+/K+ phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |