3G3A
Structure of a lamprey variable lymphocyte receptor in complex with a protein antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.299, 133.651, 90.439 |
Unit cell angles | 90.00, 114.69, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.22211 |
Rwork | 0.220 |
R-free | 0.27068 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g39 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.755 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.117 | 0.252 |
Number of reflections | 88576 | |
<I/σ(I)> | 12.6 | 3.2 |
Redundancy | 4.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.6 | 296 | 2.0 M ammonium sulfate and 0.1 M sodium citrate, pH 5.6, EVAPORATION, temperature 296K |