3G2W
VHS Domain of human GGA1 complexed with a DXXLL hinge peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-04-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.044 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.800, 72.900, 103.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.289 - 2.400 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jwf |
RMSD bond length | 0.012 |
RMSD bond angle | 1.431 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.130 | 0.544 |
Number of reflections | 14702 | |
<I/σ(I)> | 15.8 | 4.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.09 | 10.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 15%(w/v) PEG 5000 mmE, 0.2M NH4I, 0.3M 1,6-hexanediol, 0.1M MES-NaOH, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |