3G2N
Crystal structure of N-acylglucosylamine with glycogen phosphorylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.639, 128.639, 116.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.19857 |
| Rwork | 0.197 |
| R-free | 0.22734 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.044 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.478 |
| Number of reflections | 56904 | |
| <I/σ(I)> | 14.2 | 5.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 292 | pH 6.8, SMALL TUBES, temperature 292K |
