3G21

Crystal structure of the C-terminal domain of the Rous Sarcoma Virus capsid protein: Low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.85503
Spacegroup name	P 32 2 1
Unit cell lengths	32.179, 32.179, 113.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.000 - 0.900
R-factor	0.124
Rwork	0.124
R-free	0.14200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g1g
RMSD bond length	0.018
RMSD bond angle	0.035
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	0.930
High resolution limit [Å]	0.900	0.900
Rmerge	0.058	0.167
Number of reflections	46299
<I/σ(I)>	36.2	7.2
Completeness [%]	89.0	30
Redundancy	11.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.3	291	0.20M Succinic acid/KOH, pH4.3, 13-18% PEG8000, 0.75M Magnesium Nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K