3G19

The structure of the Caulobacter crescentus clpS protease adaptor protein in complex with LLL tripeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	28.658, 34.419, 71.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.388 - 1.849
R-factor	0.188
Rwork	0.185
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.590
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.043	0.028	0.116
Rmeas	0.043
Total number of observations	33923
Number of reflections	5661
<I/σ(I)>	16.2
Completeness [%]	87.1	99.3	51.4
Redundancy	6	6.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	300	0.1 M bis-tris pH 5.5, 0.2 M MgCl2, 25% PEG 3350, vapor diffusion, temperature 300K