3G13
Crystal structure of putative conjugative transposon recombinase from Clostridium difficile
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.470, 71.318, 94.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.840 - 2.000 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23918 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.840 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | 0.459 |
Number of reflections | 29582 | |
<I/σ(I)> | 10.1 | 2.86 |
Completeness [%] | 86.1 | |
Redundancy | 11 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris pH 6.5, 28% PEG monomethylether 2000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |