3G12
Crystal structure of a putative lactoylglutathione lyase from Bdellovibrio bacteriovorus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2007-10-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.276, 91.276, 57.711 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.580 |
R-factor | 0.21392 |
Rwork | 0.212 |
R-free | 0.27481 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.219 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.106 | 0.661 |
Number of reflections | 8394 | |
<I/σ(I)> | 5.4 | 1.5 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 4.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM Bis-tris pH 6.5, 2M Ammonium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |