3G10
Structure of S. pombe Pop2p - Mg2+ and Mn2+ bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.271 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.124, 53.991, 95.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.823 - 2.597 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p51 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.823 | 2.690 |
| High resolution limit [Å] | 2.597 | 2.597 |
| Rmerge | 0.076 | 0.340 |
| Number of reflections | 8371 | |
| <I/σ(I)> | 20.168 | 2.6 |
| Completeness [%] | 93.2 | 57.6 |
| Redundancy | 6.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 18% PEG 8000, 200mM magnesium acetate, 100mM MES, pH 6.5, VAPOR DIFFUSION, temperature 277K |
