3G0S
Dihydrodipicolinate synthase from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-23 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.817, 56.273, 85.801 |
| Unit cell angles | 90.00, 120.36, 90.00 |
Refinement procedure
| Resolution | 32.400 - 1.850 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pur |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.550 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.051 | 0.581 |
| Number of reflections | 50958 | |
| <I/σ(I)> | 22.332 | 1.99 |
| Completeness [%] | 97.2 | 96.7 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Magnesium chloride, 0.1 M Cacodylate buffer, 10% PEG 3000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
