3G06
The Salmonella Virulence Effector SspH2 Functions As A Novel E3 Ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-07-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.885, 76.885, 281.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.22094 |
Rwork | 0.219 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.936 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 63141 | |
<I/σ(I)> | 23 | 2.2 |
Completeness [%] | 98.8 | 95.8 |
Redundancy | 10.5 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 8-10% PEG5K, 5% tacsimate, 0.1M HEPES pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |