3FZF
Crystal Structure of Hsc70/Bag1 in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.289, 121.123, 52.773 |
| Unit cell angles | 90.00, 106.25, 90.00 |
Refinement procedure
| Resolution | 24.870 - 2.200 |
| R-factor | 0.2074 |
| Rwork | 0.200 |
| R-free | 0.29235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hx1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.648 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.870 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.114 | 0.539 |
| Number of reflections | 32145 | |
| <I/σ(I)> | 3.3 | 1.2 |
| Completeness [%] | 98.2 | 97.4 |
| Redundancy | 3.9 | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
