3FZ7
Crystal structure of apo glutamate decarboxylase beta from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-07 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9791 |
Spacegroup name | P 32 |
Unit cell lengths | 115.564, 115.564, 206.561 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.154 |
Rwork | 0.151 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.694 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 106626 |
Completeness [%] | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | 2.0 M Ammonium sulfate, 0.1 M Tris-HCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |