3FZ2
Crystal structure of the tail terminator protein from phage lambda (gpU-D74A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.370, 125.400, 211.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 2.700 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.450 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.840 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.083 | 0.289 |
| Number of reflections | 123255 | |
| <I/σ(I)> | 8.8 | 3.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.57 | 4.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2M Di-ammonium tartrate, 2.2M Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
