3FZ0
Inosine-Guanosine Nucleoside Hydrolase (IG-NH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97565 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 124.690, 116.040, 204.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.500 |
| R-factor | 0.19102 |
| Rwork | 0.189 |
| R-free | 0.22433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q8f |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.130 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Total number of observations | 374972 | |
| Number of reflections | 51751 | |
| <I/σ(I)> | 16.1 | 4.7 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 200mM ammonium sulfate, 22% PEG MME 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
