3FYN
Crystal structure from the mobile metagenome of Cole Harbour Salt Marsh: Integron Cassette Protein HFX_CASS3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-13 |
Detector | SBC-3 |
Wavelength(s) | 0.97923 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.827, 62.374, 37.247 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.985 - 1.449 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.20700 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.904 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.449 | 1.449 |
Rmerge | 0.078 | 0.696 |
Number of reflections | 23542 | |
<I/σ(I)> | 24.992 | 1.906 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.2 M Magnesium acetate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |