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3FYN

Crystal structure from the mobile metagenome of Cole Harbour Salt Marsh: Integron Cassette Protein HFX_CASS3

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-BM
Synchrotron siteAPS
Beamline19-BM
Temperature [K]100
Detector technologyCCD
Collection date2008-10-13
DetectorSBC-3
Wavelength(s)0.97923
Spacegroup nameP 21 21 2
Unit cell lengths57.827, 62.374, 37.247
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution27.985 - 1.449
R-factor0.181
Rwork0.179
R-free0.20700
Structure solution methodSAD
RMSD bond length0.004
RMSD bond angle0.904
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHENIX
Refinement softwarePHENIX
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.530
High resolution limit [Å]1.4491.449
Rmerge0.0780.696
Number of reflections23542
<I/σ(I)>24.9921.906
Completeness [%]99.999.9
Redundancy4.24.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2940.2 M Magnesium acetate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K

217705

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