3FYM
The 1A structure of YmfM, a putative DNA-binding membrane protein from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Collection date | 2006-02-02 |
| Wavelength(s) | 2.07 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.456, 45.456, 72.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| R-factor | 0.11 |
| Rwork | 0.110 |
| R-free | 0.14000 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.038 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 38933 | |
| Completeness [%] | 92.5 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M MES pH6.5, 0.01M Zinc Sulphate, 25% PEG 550MME, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
