3FYH
Recombinase in complex with ADP and metatungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 61 |
| Unit cell lengths | 83.160, 83.160, 106.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | LSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.420 |
| Number of reflections | 32232 | |
| <I/σ(I)> | 12.9 | 3.5 |
| Completeness [%] | 97.7 | 89.6 |
| Redundancy | 11.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 0.05 M magnesium chloride, 0.4 M Na CL, 0.05 M TRIS, 7% PEG 3350, 0.5 mM Na6H2W12O40, pH 7.90, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
