3FYF
Crystal structure of uncharacterized protein bvu_3222 from bacteroides vulgatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.959, 62.249, 67.051 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.24308 |
| Rwork | 0.242 |
| R-free | 0.27689 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.044 | 0.770 |
| Number of reflections | 19563 | |
| <I/σ(I)> | 9.8 | 1.3 |
| Completeness [%] | 99.0 | 98.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 200MM POTASSIUM CHLORIDE, PH 7.5, 20% PEG3350, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
