3FY6
Structure from the mobile metagenome of V. Cholerae. Integron cassette protein VCH_CASS3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.332, 97.398, 115.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.867 - 2.100 |
| R-factor | 0.1728 |
| Rwork | 0.170 |
| R-free | 0.22380 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.430 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.088 | 0.403 |
| Number of reflections | 33584 | |
| <I/σ(I)> | 9.4 | 3 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 0.1M sodium acetate , 2.0M sodium Formate, pH 4.6, VAPOR DIFFUSION |
