3FXQ
Crystal structure of the LysR-type transcriptional regulator TsaR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.496, 51.489, 107.754 |
Unit cell angles | 90.00, 110.35, 90.00 |
Refinement procedure
Resolution | 65.290 - 1.850 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21300 |
Structure solution method | MIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.353 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.020 | 1.940 |
High resolution limit [Å] | 1.849 | 1.849 |
Rmerge | 0.029 | 0.131 |
Number of reflections | 59898 | |
<I/σ(I)> | 25.08 | 6.16 |
Completeness [%] | 99.1 | 97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 4.6 | 298 | 2M Na formate, 0.1M Na acetate pH 4.6, microbatch under oil, temperature 298K |
1 | microbatch under oil | 4.6 | 298 | 2M Na formate, 0.1M Na acetate pH 4.6, microbatch under oil, temperature 298K |
1 | microbatch under oil | 4.6 | 298 | 2M Na formate, 0.1M Na acetate pH 4.6, microbatch under oil, temperature 298K |