3FXQ
Crystal structure of the LysR-type transcriptional regulator TsaR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.496, 51.489, 107.754 |
| Unit cell angles | 90.00, 110.35, 90.00 |
Refinement procedure
| Resolution | 65.290 - 1.850 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21300 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.353 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.020 | 1.940 |
| High resolution limit [Å] | 1.849 | 1.849 |
| Rmerge | 0.029 | 0.131 |
| Number of reflections | 59898 | |
| <I/σ(I)> | 25.08 | 6.16 |
| Completeness [%] | 99.1 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 4.6 | 298 | 2M Na formate, 0.1M Na acetate pH 4.6, microbatch under oil, temperature 298K |
| 1 | microbatch under oil | 4.6 | 298 | 2M Na formate, 0.1M Na acetate pH 4.6, microbatch under oil, temperature 298K |
| 1 | microbatch under oil | 4.6 | 298 | 2M Na formate, 0.1M Na acetate pH 4.6, microbatch under oil, temperature 298K |
