3FXK

Crystal Structure of Human Protein phosphatase 1A (PPM1A) Bound with Phosphate at 10 mM of Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-03-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	90.760, 90.760, 105.711
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.198
Rwork	0.196
R-free	0.23400
RMSD bond length	0.010
RMSD bond angle	1.272
Data scaling software	SCALA (3.2.25)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.832	27.830	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge	0.065	0.029	0.353
Total number of observations		8889	41122
Number of reflections	29451
<I/σ(I)>	10.18	18.6	2.2
Completeness [%]	98.8	98.3	98.3
Redundancy	9.6	8.7	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	277	50mM potassium phosphate, 12%(w/v) PEG8000, 15%(v/v) glycerol, 2mM DTT, 10mM MnCl2, pH 5.2, vapor diffusion, hanging drop, temperature 277K