3FXK
Crystal Structure of Human Protein phosphatase 1A (PPM1A) Bound with Phosphate at 10 mM of Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.760, 90.760, 105.711 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23400 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.272 |
Data scaling software | SCALA (3.2.25) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.832 | 27.830 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.065 | 0.029 | 0.353 |
Total number of observations | 8889 | 41122 | |
Number of reflections | 29451 | ||
<I/σ(I)> | 10.18 | 18.6 | 2.2 |
Completeness [%] | 98.8 | 98.3 | 98.3 |
Redundancy | 9.6 | 8.7 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 50mM potassium phosphate, 12%(w/v) PEG8000, 15%(v/v) glycerol, 2mM DTT, 10mM MnCl2, pH 5.2, vapor diffusion, hanging drop, temperature 277K |