3FXJ
Crystal Structure of Human Protein phosphatase 1A (PPM1A) Bound with Phosphate at 3 mM of Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.382, 91.382, 106.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.970 - 2.500 |
| R-factor | 0.194 |
| Rwork | 0.190 |
| R-free | 0.25500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.366 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (7.1SSI) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 14.970 | 14.970 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.310 | 2.500 |
| Rmerge | 0.161 | 0.072 | 0.389 |
| Total number of observations | 11226 | 10921 | |
| Number of reflections | 17977 | ||
| <I/σ(I)> | 9.7 | 1.9 | |
| Completeness [%] | 98.7 | 94.7 | 100 |
| Redundancy | 5.84 | 5.47 | 6.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 50mM potassium phosphate, 12%(w/v) PEG8000, 15%(v/v) glycerol, 2mM DTT, 3mM MnCl2, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
