3FXH
Crystal structure from the mobile metagenome of Halifax Harbour Sewage Outfall: Integron Cassette Protein HFX_CASS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-13 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 40.744, 66.829, 48.873 |
| Unit cell angles | 90.00, 113.52, 90.00 |
Refinement procedure
| Resolution | 26.787 - 1.837 |
| R-factor | 0.191 |
| Rwork | 0.186 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.924 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.837 | 1.837 |
| Rmerge | 0.087 | 0.669 |
| Number of reflections | 10187 | |
| <I/σ(I)> | 35.289 | 3.018 |
| Completeness [%] | 97.3 | 95.8 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.2 M Tri-lithium citrate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
