3FXE
Crystal structure of interacting domains of IcmR and IcmQ (seleno-derivative)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950, 0.97976, 0.95003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.090, 42.100, 78.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.040 | 0.130 |
| Number of reflections | 10087 | |
| <I/σ(I)> | 23.9 | 18 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 7.7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 295 | 100 mM Na acetate pH 4.7, 30% PEG 1500, 100 mM L-cysteine, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
