3FX7
Crystal structure of hypothetical protein of HP0062 from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.23985 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.303, 51.895, 83.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.650 |
R-factor | 0.18828 |
Rwork | 0.186 |
R-free | 0.22512 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gts |
RMSD bond length | 0.010 |
RMSD bond angle | 1.125 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.049 | 0.248 |
Number of reflections | 20637 | |
<I/σ(I)> | 59.33 | 10.97 |
Completeness [%] | 97.1 | 95.1 |
Redundancy | 12.8 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5% PEG 3350, 100mM citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |