3FX3
Structure of a putative cAMP-binding regulatory protein from Silicibacter pomeroyi DSS-3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97926,0.97940 |
| Spacegroup name | H 3 |
| Unit cell lengths | 137.728, 137.728, 94.626 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.540 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.422 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.079 | 0.491 |
| Number of reflections | 33872 | |
| <I/σ(I)> | 35.955 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.49M NaH2PO4, 0.91M K2HPO4, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
