3FX0
Crystal structure of Human NEMO CC2_LZ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2008-08-03 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 65 |
| Unit cell lengths | 76.194, 76.194, 76.892 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 3.200 |
| Rwork | 0.247 |
| R-free | 0.30600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.990 | 35.000 | 3.400 |
| High resolution limit [Å] | 3.190 | 5.810 | 3.200 |
| Rmerge | 0.058 | 0.039 | 0.546 |
| Number of reflections | 4241 | ||
| Completeness [%] | 99.0 | 96.5 | 99.4 |
| Redundancy | 5.4 | 4.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 10% PEG 1000, 10% PEG 4000, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
