3FWU
Crystal structure of Leishmania major MIF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97650 |
| Spacegroup name | H 3 |
| Unit cell lengths | 52.321, 52.321, 97.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.540 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uiz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.121 |
| Number of reflections | 9160 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.8 | 93.4 |
| Redundancy | 5.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 30% w/v PEG 4000, 100mM Imidazole, pH 6.5, Hanging drop, temperature 290K, VAPOR DIFFUSION, HANGING DROP |
