3FWL
Crystal Structure of the Full-Length Transglycosylase PBP1b from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97882, 0.97899, 0.96358 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.245, 296.997, 62.824 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.257 - 3.086 |
R-factor | 0.2145 |
Rwork | 0.211 |
R-free | 0.27330 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.082 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.086 |
Number of reflections | 21677 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 20mM Tris, 300mM NaCl, 0.28mM n-Dodecyl-N,N-dimethylamine-N-oxide (LDAO), 1.2M sodium formate, 0.01M beta-Nicotinamide adenine dinucleotide hydrate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |