3FWK
Crystal Structure of Candida glabrata FMN Adenylyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 80.090, 80.090, 78.089 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.200 |
R-factor | 0.162 |
Rwork | 0.162 |
R-free | 0.17800 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.621 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 90728 | |
<I/σ(I)> | 48.224 | 2.347 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 11.4 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 6% (w/v) PEG 4000, 0.1 M Sodium acetate, pH 4.6,Temperature 289K, VAPOR DIFFUSION, HANGING DROP |