3FWK
Crystal Structure of Candida glabrata FMN Adenylyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.090, 80.090, 78.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.200 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.17800 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.621 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 90728 | |
| <I/σ(I)> | 48.224 | 2.347 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 11.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 6% (w/v) PEG 4000, 0.1 M Sodium acetate, pH 4.6,Temperature 289K, VAPOR DIFFUSION, HANGING DROP |
