3FWJ

Ferric camphor bound Cytochrome P450cam containing a selenocysteine as the 5th heme ligand, orthorombic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99187
Spacegroup name	P 21 21 21
Unit cell lengths	63.100, 65.000, 105.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.380 - 1.900
R-factor	0.185
Rwork	0.183
R-free	0.23100
Starting model (for MR)	1akd
RMSD bond length	0.013
RMSD bond angle	1.499
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.380	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.047	0.343
Number of reflections	34678	4676
<I/σ(I)>		3.4
Completeness [%]	99.0	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20 % glycerol for cryoprotection, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K