3FWG
Ferric camphor bound Cytochrome P450cam, Arg365Leu, Glu366Gln, monoclinic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9994 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.180, 62.795, 95.605 |
Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
Resolution | 39.130 - 1.550 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.21700 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.367 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.130 | 1.650 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.056 | 0.347 |
Number of reflections | 111321 | 17853 |
<I/σ(I)> | 3.8 | |
Completeness [%] | 96.4 | 90.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 24% PEG 4000, 50 mM Tris HCl, 250 mM KCl, 20% glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |