3FWF
Ferric camphor bound cytochrome P450cam containing a Selenocysteine as the 5th heme ligand, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.520, 61.930, 94.460 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 45.640 - 1.830 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20700 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.529 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.065 | 0.388 |
| Number of reflections | 68754 | 10086 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 24 % PEG 4000, 250 mM KCl, 50 mM TrisHCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
