3FWE
Crystal Structure of the Apo D138L CAP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2007-11-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.996, 67.996, 230.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.650 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.27700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 2.357 |
| High resolution limit [Å] | 2.300 | 2.297 |
| Rmerge | 0.081 | 0.417 |
| Number of reflections | 24422 | |
| <I/σ(I)> | 7.199 | |
| Completeness [%] | 67.1 | 64.8 |
| Redundancy | 1.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 4000 and 200mM Proline, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
